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nebuilder hifi dna assembly cloning kit  (New England Biolabs)


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    Structured Review

    New England Biolabs nebuilder hifi dna assembly cloning kit
    Nebuilder Hifi Dna Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebuilder+hifi+dna+assembly+kit/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pmc13626086-372-8-8
    Average 99 stars, based on 3215 article reviews
    nebuilder hifi dna assembly cloning kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Engineering Thermobifida fusca cutinase variants for the sustainable recycling of PET from cotton-blended textiles
    Article Snippet: The vector was linearized by digestion with EcoRI-HF and XhoI (New England Biolabs). .. Recombinant plasmids were constructed using the NEBuilder HiFi DNA Assembly Kit following the manufacturer’s instructions. ..

    Construct:

    Article Title: Engineering Thermobifida fusca cutinase variants for the sustainable recycling of PET from cotton-blended textiles
    Article Snippet: The vector was linearized by digestion with EcoRI-HF and XhoI (New England Biolabs). .. Recombinant plasmids were constructed using the NEBuilder HiFi DNA Assembly Kit following the manufacturer’s instructions. ..

    Clone Assay:

    Article Title: Trifluoroacetate mediated reduction of atherosclerosis in mice
    Article Snippet: Beta-galactosidase was used to normalize the luciferase signal by co-transfection with pSV-β-galactosidase control vector (Promega). .. All three PPAR isoform cDNAs were cloned into the HindIII site of pBApo-CMV plasmid (Takara) by NEBuilder HiFi DNA Assembly kit (NEB). ..

    Article Title: RNase-mediated defence and its association with SCCmec in Staphylococcus aureus.
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli-S. aureus shuttle vector pLI50 plasmid 23 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software (http://www.softberry.com/berry.phtml/).

    Article Title: RNase-mediated defence and its association with SCC mec in Staphylococcus aureus
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli - S. aureus shuttle vector pLI50 plasmid using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software ( http://www.softberry.com/berry.phtml/ ).

    Plasmid Preparation:

    Article Title: Trifluoroacetate mediated reduction of atherosclerosis in mice
    Article Snippet: Beta-galactosidase was used to normalize the luciferase signal by co-transfection with pSV-β-galactosidase control vector (Promega). .. All three PPAR isoform cDNAs were cloned into the HindIII site of pBApo-CMV plasmid (Takara) by NEBuilder HiFi DNA Assembly kit (NEB). ..

    Article Title: Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
    Article Snippet: pCAG rabies packaging plasmids were created by performing PCR on the CVS-N2c- Δ G-mCherry plasmid (Addgene plasmid #73464) to amplify the N, P and L gene sequences, while the G gene was amplified from N2A-N2cG cells (a gift from Dr. Andrew Murray). .. The gene fragments were then inserted into the pCAG-T7-polymerase plasmid backbone (Addgene plasmid #59926) using a NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520). ..

    Article Title: Developing a 4-Helix Bundle Protein Engineering Platform for Isolating Rare-Earth Elements
    Article Snippet: .. All gBlocks were designed with a 20-basepair overhang with the linearized vector for compatibility with the NEBuilder HiFi DNA Assembly kit (New England Biolabs) for streamlined insertion of the designed 4HB genes (Table S2) into linearized pET-28a. .. Constructs were transformed into E. coli DH5α cells purchased from New England Biolabs.

    Article Title: Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy
    Article Snippet: .. Individual point mutants were generated via site-directed mutagenesis using the NEBuilder HiFi DNA assembly kit and confirmed via whole plasmid sequencing. .. The expression vector was midi-prepped (Zymo Research), linearized with SpeI (NEB), cleaned with SureClean (Meridian Bioscience), and used for in vitro transcription with Hi-T7 (NEB).

    Article Title: RNase-mediated defence and its association with SCCmec in Staphylococcus aureus.
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli-S. aureus shuttle vector pLI50 plasmid 23 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software (http://www.softberry.com/berry.phtml/).

    Article Title: RNase-mediated defence and its association with SCC mec in Staphylococcus aureus
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli - S. aureus shuttle vector pLI50 plasmid using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software ( http://www.softberry.com/berry.phtml/ ).

    Generated:

    Article Title: Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy
    Article Snippet: .. Individual point mutants were generated via site-directed mutagenesis using the NEBuilder HiFi DNA assembly kit and confirmed via whole plasmid sequencing. .. The expression vector was midi-prepped (Zymo Research), linearized with SpeI (NEB), cleaned with SureClean (Meridian Bioscience), and used for in vitro transcription with Hi-T7 (NEB).

    Mutagenesis:

    Article Title: Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy
    Article Snippet: .. Individual point mutants were generated via site-directed mutagenesis using the NEBuilder HiFi DNA assembly kit and confirmed via whole plasmid sequencing. .. The expression vector was midi-prepped (Zymo Research), linearized with SpeI (NEB), cleaned with SureClean (Meridian Bioscience), and used for in vitro transcription with Hi-T7 (NEB).

    Sequencing:

    Article Title: Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy
    Article Snippet: .. Individual point mutants were generated via site-directed mutagenesis using the NEBuilder HiFi DNA assembly kit and confirmed via whole plasmid sequencing. .. The expression vector was midi-prepped (Zymo Research), linearized with SpeI (NEB), cleaned with SureClean (Meridian Bioscience), and used for in vitro transcription with Hi-T7 (NEB).

    Amplification:

    Article Title: RNase-mediated defence and its association with SCCmec in Staphylococcus aureus.
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli-S. aureus shuttle vector pLI50 plasmid 23 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software (http://www.softberry.com/berry.phtml/).

    Article Title: RNase-mediated defence and its association with SCC mec in Staphylococcus aureus
    Article Snippet: .. The amplified sadR gene was cloned into the E. coli - S. aureus shuttle vector pLI50 plasmid using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA, Catalogue: E2621S). .. The promoter region was predicted using Softberry software ( http://www.softberry.com/berry.phtml/ ).



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